Modifying Enzymes
FUJIFILM Biosciences Cholesterol Esterase | 9026-00-0 | MFCD00071052 | 1000u
Cholesterol Esterase | 9026-00-0 | MFCD00071052 | 1000u
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase from bovine liver ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret) | 9054-89-1 | MFCD00132404 | 15KU
Superoxide Dismutase from bovine liver ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret) | 9054-89-1 | MFCD00132404 | 15KU
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
New England Biolabs, Inc. Bacteroides Heparinase III – 35 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase from Clostridium perfringens (C. welchii) Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 9001-67-6 | MFCD00131711 | 250UN
Neuraminidase from Clostridium perfringens (C. welchii) Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder | 9001-67-6 | MFCD00131711 | 250UN
New England Biolabs, Inc. Endoproteinase AspN – 50 µg
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues.
AVIDITY LLC BIO-500 Biotin Solution, 5 mM d-biotin, 10 mL, 10 mM Bicine buffer, pH 8.3, Filter sterilized (0.22 μm), Room temperature shipping, -20 °C storage
The AVIDITY LLC BIO500 is a 5mM d-biotin solution supplied in a 10mL volume, designed for critical molecular biology applications. It is particularly suitable for in-vivo biotinylation culture inductions utilizing pBirAcm BirA over-expression plasmids and AviTag™-protein fusion vectors. The solution is also appropriate for large-scale BirA reactions requiring supplemental d-biotin and for supplementing induction growth media. Filter sterilized through a 0.22μm filter, this product ensures sterility for sensitive scientific protocols.
- Concentration: 5 mM d-biotin
- Volume: 10 mL
- Buffer: 10 mM Bicine
- pH: 8.3
- Sterility: Filter Sterilized (0.22 μm)
- Shipping Condition: Room temperature
- Storage Temperature: -20 °C
- Primary Application: In-vivo biotinylation
- Secondary Application: Large scale BirA reactions
Sigma Aldrich Fine Chemicals Biosciences Protease from bovine pancreas Type I, >=5 units/mg solid | 9001-92-7 | MFCD00132092 | 250MG
Protease from bovine pancreas Type I, >=5 units/mg solid | 9001-92-7 | MFCD00132092 | 250MG
Supply Solutions Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)
Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)
Takara Bio RNASE A 100MG
Rnasea (Lyophilized) 100Mg. The Rnase A Is Primarily Intended To Be Used With Nucleospin And Nucleobond Kits.
Cayman Chemical N-Oleoyl ProlIn 10mg
An endogenous N-acyl amine that is decreased in lumbar spinal cord after capsaicin administration (1 UG/10 µl) and has surfactant properties (critical micelle concentration = 4.8 µM)
Medchemexpress LLC Fibrinogen (bovine) | 9001-32-5 | 92.0% | ≈340 kDa | 100MG
Bovine fibrinogen is a plasma glycoprotein supplied as a white to off-white powder for research applications in coagulation, thrombosis, and vascular biology. It is converted to fibrin by thrombin and is used in in vitro clotting assays and studies of inflammatory signaling in endothelial cells.
- White to off-white powder; soluble plasma glycoprotein.
- Used to study blood clotting, fibrin formation, thrombosis, and vascular inflammation.
- Activated by thrombin to assemble fibrin clots.
- Suitable for in vitro clotting assays and endothelial cell signaling studies.
- Stored per certificate of analysis; typically kept frozen for long-term storage (e.g., -20°C).
- Documentation available: datasheet, certificate of analysis, safety data sheet, and handling instructions.
- CAS number: 9001-32-5.
New England Biolabs, Inc. Mung Bean Nuclease – 1500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
New England Biolabs, Inc. E.coli DNA Ligase – 200 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
Medchemexpress LLC α-Amylase, Human Saliva | 9000-90-2 | 1 KU
α-Amylase, Human Saliva is a hydrolase enzyme isolated from human saliva. It functions by catalyzing the hydrolysis of internal α-1,4-glycosidic linkages in starch, leading to the production of compounds such as glucose and maltose. This enzyme is a valuable tool for various life science research applications, particularly in the study of starch metabolism and in research focused on developing nutraceuticals for conditions like diabetes and obesity.
- Isolated from human saliva
- Catalyzes hydrolysis of internal α-1,4-glycosidic linkages in starch
- Yields products like glucose and maltose
- Useful in life science research
- Can be used in the study of starch metabolism
- Can be used in research aimed at developing nutraceuticals for diabetes and obesity